ELISA Test / Chemiluminescence

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Elisa test is the gold standard test to quantify SARS-CoV-2 antibodies, because of its sensitivity and specificity.

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What is it ELISA test / Chemiluminescence?

Serological test as ELISA/ chemiluminescence are high performance automated trials for the semi-quantitative determinatin of IgG and IgM antibodies against SARS-Cov-2 in blood samples. Its function is to detect immune response of our body. Nowadays these are the test for anibodies levels quantification anti SARS-Cov-2, because of its sensitivity and specificity.

ELISA test is performed through blood collection. Blood is analysed first of all by immunochemiluminescence technique, that defines if the patient has or not SARS-Cov-2 virus antibodies.

Once immunochemiluminescence is performed, in case it is positive, the sample is analysed to define IgG and IgM antibodies level in the body.

Do I have immunity against COVID-19?

There is correlation between antibodies presence and protection level against the disease. However, for now there are no conclusive studies about neutralizing IgG antbodies level fo SARS-Cov-2 or about the time it remain in our body.

What are IgM and IgG antibodies?

IgM is the first antibody produced when there is an infection, considered a marker of the acute stage of the disease.

IgG are produced later, maybe also present on the acute stage of the infection. IgG remain circulating on the blood, to protect the person against future infections.

When do IgM and IgG antibodies appear? What is the window period?

IgM antibody can be detected approximately from the 7th day after the infection. Generally, the body produce IgG persistent antibodies from the 14th day since the infection.

ELISA serological test results chart

Total antibodies < 0,9 IgM < 0,9 IgG < 0,9*	IgM > 1,1 IgG < 0,9*	IgM > 1,1 IgG > 1,1*	IgM < 0,9 IgG > 1,1*
There is no antibodies presence	IgM antibodies presence	IgM and IgG antibodies presence	IgG antibodies presence
Not immunised	Not immunised	Immunised	Immunised
1.- Didn't have the infection 2.- Had the infection, but antibodies disappeared 3.- You are in window period*	Probably infected	Recently had the infection and is in remission stage	I overcame the infection
If you've been in close contact with a positive case or have disease symptoms we recommend you to have a PCR test	We recommend you isolation and PCR test to confirm diagnosis	We recommend you to repeat IgG antibodies in 3 months to follow-up antibodies levels	We recommend you to repeat IgG antibodies in 3 months to follow-up antibodies levels

Results between 0,9 and 1,1 are considered indeterminate. Probably you are in seroconversion period (antibodies levels modification). Please check with a physician to know the results. In these cases, it is recommended to repeat antibodies study in a week.

* Time period when a person is infected and that the infection can be detected (in case of COVID-19 around 10 days)

Where can I have the test?

CENTER	SCHEDULE
Valencia	24h Every day from 8:00 till 20:00, go to the adjacent building next to the hospital. Off this schedule go to emergency service. Av. de la Ilustración, 1, 46100 Burjassot, Valencia
Elche	24h Monday to Friday from 9:00 till 14:00 and from 16:00 till 19:00, access through the outpatient entrance. Off this schedule go to emergency service. Calle Max Planck, 3, 03203 Elche, Alicante
Benidorm	24h From 8:00 till 20:00 access 1st floor. Off this schedule go to emergency service. Calle Dr. Santiago Ramón y Cajal, 7, 03503 Benidorm, Alicante
Gandía	From Monday to Saturday from 8:00 till 19:00. Sunday from 10:00 till 19:00. Carrer de Daimús, 26, 46701 Gandia, Valencia
Teulada	Every day from 8:00 till 19:00. Ctra. Nacional 332. Kilómetro 181,5. Partida, el Pla, 03725 Teulada, Alicante
Murcia	Every day from 8:00 till 16:00. Av. Nueva Condomina, 11, 30110 Churra, Murcia
Torrevieja	Every day from 8:30 till 18:00. Centro Comercial Ozone, Avda. De Rosa Mazón Valero, s/n, 03184 Torrevieja, Alicante
Alcoy	From Monday to Friday from 8:00 till 17:00. Saturday and Sunday from 8:00 till 20:00. Carrer Oliver, 55, 03802 Alcoi, Alicante